R-024. Cryopreservation of a few spermatozoa in hamster zonae pellucidae
نویسندگان
چکیده
منابع مشابه
Human spermatozoa attach to trypsin-treated hamster zonae pellucidae but do not undergo acrosome reactions.
The acrosome reaction by spermatozoa is an indispensable prerequisite for fertilization, and zonae pellucidae of human oocytes induce this reaction in the human spermatozoa attached to them. Human spermatozoa can attach to the zona pellucida of hamster oocytes when the oocytes have been treated with trypsin. We examined whether or not trypsin-treated hamster zona pellucida induces the acrosome ...
متن کاملPersistence of anti-zonae pellucidae antibodies following a single inoculation of porcine zonae pellucidae in the domestic equine.
In this study of equids, we investigated the antibody response and the effect on the estrous cycle following a single inoculation of porcine zonae pellucidae (pZP) employing controlled-release methodology. We also investigated the use of two different water-soluble adjuvants as an alternative to oil-based adjuvants. Twenty-seven domestic mares were inoculated with various formulations of pZP an...
متن کاملInvolvement of trypsin-like activity in binding of mouse spermatozoa to zonae pellucidae.
Work from a number of laboratories has shown that fertilization is blocked in the presence of protease inhibitors, although the specific site of inhibition has not been identified. The present experiments were designed to discriminate between sperm binding to zonae pellucidae as opposed to sperm penetration through zonae, so as to assess the effect of protease inhibitors on these two distinct e...
متن کاملModerate heat treatment increases the penetrability of zonae pellucidae of salt-stored mammalian oocytes by spermatozoa.
The zona pellucida of mammalian oocytes stored in highly concentrated solutions of neutral salts is known to retain its biological and biochemical properties. However, the zona may become resistant to sperm penetration as the storage period is increased. In cattle and hamsters, the penetrability of zonae of salt-stored oocytes was restored or increased by treating the oocytes with moderate heat...
متن کاملAbnormal zonae pellucidae in mice lacking ZP1 result in early embryonic loss.
All vertebrates have an egg shell that surrounds ovulated eggs and plays critical roles in gamete recognition. This extracellular matrix is known as the zona pellucida in eutherian mammals and consists of three glycoproteins, ZP1, ZP2 and ZP3 in the mouse. To investigate the role of ZP1 in fertilization and early development, we have used targeted mutagenesis in embryonic stem cells to create m...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Human Reproduction
سال: 1999
ISSN: 1460-2350,0268-1161
DOI: 10.1093/humrep/14.suppl_3.289-a